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. 2016 Jan 1;12(1):120–132. doi: 10.7150/ijbs.12852

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DCs pulsed with PDT-LLCs induce tumor-specific immunity. Bone marrow-derived DCs were pulsed with LLC cells undergone different treatment (untreated, CNTR; freeze/thawed, F/T; or treated with a high PDT dose) and then used for the stimulation of autologous T cells for 7 days. Spleen lymphocytes these mice without any treatment were used as the control. The number of IFN-γ producing CD8+ T cells was analyzed by flow cytometry. (A) shows representative staining of 5 independent experiments, while (D) shows a summary. The frequency of CD4+CD25+FoxP3+ cells was analyzed to determine the Treg cell population. (B), and (C) are representatives of 5 independent experiments. Data in (E) and (F) show a summary of 5 independent experiments. *p<0.05, **p< 0.01, ***p<0.001; #p<0.05, ##p<0.01 versus control.