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. Author manuscript; available in PMC: 2016 Nov 27.
Published in final edited form as: Vaccine. 2015 Nov 3;33(48):6570–6578. doi: 10.1016/j.vaccine.2015.10.115

Figure 2. Intranasal vaccination elicits CD4 T cells that reside exclusively in the lung parenchyma.

Figure 2

B6 mice were immunized with ID93+GLA-SE either by intramuscular injection or intranasal instillation. One month after the third immunization blood resident T cells were labeled by intravenous injection of α–CD45.2 prior to euthanasia of the animals. (A) Vaccine specific CD4 T cells in the lungs were identified by tetramer staining. (B) Intravenous CD45.2 labeling vaccine specific CD4 T cells from intramuscular (black line) or intranasal (grey fill) vaccinated animals is shown. The bar indicates the CD45.2+ gate. (C) CD69 staining of tetramer labeled cells is shown for intramuscular (black line) and intranasal (grey fill) vaccinated animals. Means ± s.d. are shown for 4-5 mice/group. The data are representative of three experiments with similar results.