Table 1.
Instrument specific parameters for the analysis of morphine, morphine-3-β-glucuronide, morphine-6-β-glucuronide, hydromorphone and normorphine.
| Analyte | Retention Time (min) | Parent mass (m/z) | Product mass (m/z) | Collision Enery (V) | S-Lens (V) |
|---|---|---|---|---|---|
| Morphine | 1.37 | 286.1 | 165.1 | 40 | 125 |
| 185.0 | 30 | 125 | |||
| Morphine-3-β-glucuronide | 0.56 | 462.2 | 165.1 | 52 | 171 |
| 286.1 | 31 | 171 | |||
| Morphine-6-β-glucuronide | 1.14 | 462.2 | 165.1 | 52 | 171 |
| 286.1 | 31 | 171 | |||
| Hydromorphone | 1.93 | 286.1 | 165.1 | 40 | 125 |
| 185.0 | 30 | 125 | |||
| Normorphine | 0.87 | 272.1 | 152.1 | 38 | 110 |
| 209.0 | 24 | 110 | |||
| Morphine-D3 | 1.37 | 289.2 | 152.1 | 60 | 126 |
| Morphine-3-β-glucuronide-D3 | 0.56 | 465.2 | 289.1 | 30 | 167 |
| Morphine-6-β-glucuronide-D3 | 1.14 | 465.2 | 289.1 | 30 | 167 |
| Hydromrphone-D6 | 1.93 | 292.1 | 185.1 | 29 | 110 |