Table 7.
Dilution study of samples that have concentrations outside of the linear range of 1,500, 2,000, and 3,000 ng/mL and require dilution factors of 2-, 3-, and 4-fold respectively.
| 1500 ng/mL | 2000 ng/mL | 3000 ng/mL | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | %CV | %BIAS | Mean | SD | %CV | %BIAS | Mean | SD | %CV | %BIAS | |
| Morphine | 719.2 | 14.3 | 2.0 | −4.1 | 638.3 | 32.4 | 5.1 | 4.3 | 823.0 | 10.7 | 1.3 | 9.7 |
| Morphine-3-β-Glucuronide | 641.4 | 15.2 | 2.4 | −14.5 | 603.7 | 39.8 | 7.1 | −9.4 | 780.4 | 44.8 | 5.5 | −10.9 |
| Morphine-6-β-Glucuronide | 688.2 | 45.2 | 6.6 | −8.2 | 620.9 | 25.4 | 4.1 | −6.9 | 755.6 | 91.9 | 11.1 | 0.7 |
| Hydromorphone | 819.5 | 43.2 | 5.3 | 9.3 | 751.9 | 21.7 | 2.9 | 12.8 | 824.3 | 37.6 | 4.6 | 9.9 |
| Normorphine | 821.7 | 31.9 | 3.8 | 9.6 | 736.0 | 48.2 | 6.5 | −4.1 | 801.8 | 26.6 | 3.3 | 6.9 |