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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mech Dev. 2015 Oct 14;138 Pt 3:279–290. doi: 10.1016/j.mod.2015.10.001

Fig. 3. Cranial neural crest cell migration and specification is unaffected in gpc4 and wnt5b mutants.

Fig. 3

(A–O) Neural crest specification appears normal within gpc4 and wnt5b mutants Scale bar = 100 µm. (A– I) Pre-migratory neural crest markers. 6 somites. (J–O) Pharyngeal arch markers. (J–L) 28 hpf. (M–O) 32 hpf. (P–R) Migration of neural crest appears to be unaffected in gpc4 and wnt5b mutants when compared to wild type fish. Tg(fli1a:EGFP)y1 embryos have neural crest derived cells labeled by GFP expression. Embryos were fixed at 33 hpf and stained with the Alcama (Zn5) antibody (red). Pharyngeal arches are numbered. Scale bar = 50 µm.