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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mech Dev. 2015 Oct 14;138 Pt 3:279–290. doi: 10.1016/j.mod.2015.10.001

Fig. 5. Craniofacal cartilage volume is reduced in gpc4 mutants while the numbers of cells is unchanged.

Fig. 5

(A–C) Wheat germ agglutinin (gray) and DAPI (blue) staining of ceratohyal elements. (D) A 3D reconstruction of 20 cells in the center of a wild type ceratohyal stained with WGA to determine cell volume. (E) There is a significant difference between the average length of the ceratohyal and symplectic elements between the wild type and mutants. All are p = < 0.001. (F) The average number of cells in ceratohyal and symplectic elements. (G) The average volume of the ceratohyal and symplectic elements. The ratio of the ceratohyal element volume is Vfr6/VWT = 0.63. (E–G) Tests of four a priori hypotheses were conducted using a Bonferroni adjusted alpha level of 0.0125 (0.05/4). * p < 0.0125. (H) The average volume of 20 cells in the center and proximal ends from three ceratohyal elements from gpc4fr6 embryos and their phenotypically wild type siblings. Tests of two a priori hypotheses were conducted using a Bonferroni adjusted alpha level of 0.025 (0.05/2). * p < 0.025. The ratio of the center chondrocyte volume is Vfr6/VWT = 0.71. The ratio of the proximal chondrocyte volume is Vfr6/VWT = 0.74. Scale bar = 20 µm.