Table 1.
Organism | Soil type | Seeding level (EPGa) | Method type | Detergent | Flotation solution (SG) | Percent recovery | Estimated processing timeb | Refs. |
---|---|---|---|---|---|---|---|---|
Ascaris | Clay | 4 | Passive flotation | Bleach (20%) | NaNO3 (1.3) | 15.3% | 2 hours | [53] |
Sandy | 20 | 24.32% | ||||||
Loamy | 20 | 25.04% | ||||||
Ascaris | NRc | 1452- 32393 |
Centrifugal flotation |
NaOH (0.5 N) |
Glucose monohydrate (1.27) |
82% | 2 days | [54] |
Ascaris | NR | 10 | Centrifugal flotation |
Tween40 (0.5%) |
NaNO3 (1.35) | 47% | <1 hour | [55] |
Tween80 (1%) |
NaNO3 (1.35) | 66.5% | <1 hour | |||||
None | ZnSO4 (1.2) | 9.5% | 3 hours | |||||
Trichuris | NR | 1452- 32393 |
Centrifugal flotation |
NaOH (0.5 N) |
Glucose monohydrate (1.27) |
80% | 2 days | [54] |
Trichuris | NR | 1 | Centrifugal flotation, recovery by adherence to 4 coverslips |
None | MgSO4 (1.28) | 96.4% | 1-2 hours | [56] |
Ancylostoma | NR | 10 | Centrifugal flotation |
Tween40 (0.5%) |
NaNO3 (1.35) | 39.33% | <1 hour | [55] |
Tween80 (1%) |
NaNO3 (1.35) | 50% | <1 hour | |||||
None | ZnSO4 (1.2) | 9% | 3 hours | |||||
Toxocara | Sandy | 2 | Centrifugal flotation, recovery by adherence to 6 coverslips |
Tween80 (NR) |
NaNO3 (1.22) | 15% | 2-3 hours | [11] |
2 | Passive flotation, recovery by adherence to 4 coverslips |
Tween40 (NR) |
ZnSO4 (1.2) | 15% | ||||
Toxocara | Sandy | 543 | Passive flotation | None | Saccharose (1.27) | 99.98% | <1 hour | [57] |
Toxocara | Silty clay | 200 | Centrifugal flotation |
NaOH (0.1 N) |
Na2Cr2O7 (1.35) | 7.5% | <1 hour | [58] |
Clay silt | 13.9% | |||||||
Sandy | 38% | |||||||
Sand | 62.5% | |||||||
Toxocara | Sandy | 200 | Centrifugal flotation, recovery by adherence to coverslip |
Tween80 (0.2%) |
ZnSO4 (1.2) | 10.75% | 2 days | [59, 60] |
NaOH (0.1 N) |
8.8% | |||||||
Toxocara | NR | 1 | Centrifugal flotation, recovery by adherence to 4 coverslips |
None | MgSO4 (1.28) | 47.2% | 1-2 hours | [56] |
Toxocara | NR | 10 | Centrifugal flotation (3 different methods: ([12, 60, 62]) |
Tween40 (0.5%) |
NaNO3 (1.35) | 46.83% | <1 hour | [55] |
Tween80 (1%) |
NaNO3 (1.35) | 71% | <1 hour | |||||
None | ZnSO4 (1.2) | 7.67% | 3 hours | |||||
Toxocara | Clay | 210 | Centrifugal flotation X 3d, recovery by adherence to 4-5 coverslips |
NaOH (0.1 N) |
ZnSO4 (1.18) | 66.19% | 1-2 hours | [62] |
Toxocara | Silt | 20 | Centrifugal flotation |
Tween80 (0.1%) |
NaCl (1.25) | 45.3% | 1-2 hours | [63] |
Sand | NaCl (1.25) | 100.8% | ||||||
Laterite | ZnSO4 (1.52) | 56.8% | ||||||
Toxocara | NRd | 0.48 | Centrifugal flotation X 4 |
Tween80 (0.0025%) |
MgSO4 (1.275) | 82.5% | 1-2 hours | [64] |
Toxocara | NR | NR | Centrifugal flotation, recovery by adherence to 2 coverslips |
Tween80 (1%) |
NaNO3 (1.35) | 69.8% | 1-2 hours | [12] |
Echinococcus | NR | 100 | Centrifugal flotation, recovery by adherence to coverslip |
Tween80 (0.05%) |
Sucrose (1.27) | 10% | 2-3 hours | [65] |
Echinococcus | Sand | NR | Centrifugal flotation, recovery by adherence to coverslip |
Tween80 (0.05%) |
ZnCl2 (1.4) | NR | 1-2 hours | [66] |
EPG - Eggs per gram.
Processing times estimated based on given times for incubation and centrifugation steps, and do not include time needed to examine the sample after recovery.
NR - not reported.
Multiple flotations performed (e.g. X 4 denotes four sequential flotations).