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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Trends Parasitol. 2015 Oct 1;31(12):625–639. doi: 10.1016/j.pt.2015.08.007

Table 1.

Reported parameters yielding maximum rates of recovery of STH from soils.

Organism Soil type Seeding level (EPGa) Method type Detergent Flotation solution (SG) Percent recovery Estimated processing timeb Refs.
Ascaris Clay 4 Passive flotation Bleach (20%) NaNO3 (1.3) 15.3% 2 hours [53]
Sandy 20 24.32%
Loamy 20 25.04%
Ascaris NRc 1452-
32393
Centrifugal
flotation
NaOH
(0.5 N)
Glucose
monohydrate
(1.27)
82% 2 days [54]
Ascaris NR 10 Centrifugal
flotation
Tween40
(0.5%)
NaNO3 (1.35) 47% <1 hour [55]
Tween80
(1%)
NaNO3 (1.35) 66.5% <1 hour
None ZnSO4 (1.2) 9.5% 3 hours
Trichuris NR 1452-
32393
Centrifugal
flotation
NaOH
(0.5 N)
Glucose
monohydrate
(1.27)
80% 2 days [54]
Trichuris NR 1 Centrifugal
flotation,
recovery by
adherence to 4
coverslips
None MgSO4 (1.28) 96.4% 1-2 hours [56]
Ancylostoma NR 10 Centrifugal
flotation
Tween40
(0.5%)
NaNO3 (1.35) 39.33% <1 hour [55]
Tween80
(1%)
NaNO3 (1.35) 50% <1 hour
None ZnSO4 (1.2) 9% 3 hours
Toxocara Sandy 2 Centrifugal
flotation,
recovery by
adherence to 6
coverslips
Tween80
(NR)
NaNO3 (1.22) 15% 2-3 hours [11]
2 Passive flotation,
recovery by
adherence to 4
coverslips
Tween40
(NR)
ZnSO4 (1.2) 15%
Toxocara Sandy 543 Passive flotation None Saccharose (1.27) 99.98% <1 hour [57]
Toxocara Silty clay 200 Centrifugal
flotation
NaOH
(0.1 N)
Na2Cr2O7 (1.35) 7.5% <1 hour [58]
Clay silt 13.9%
Sandy 38%
Sand 62.5%
Toxocara Sandy 200 Centrifugal
flotation,
recovery by
adherence to
coverslip
Tween80
(0.2%)
ZnSO4 (1.2) 10.75% 2 days [59, 60]
NaOH
(0.1 N)
8.8%
Toxocara NR 1 Centrifugal
flotation,
recovery by
adherence to 4
coverslips
None MgSO4 (1.28) 47.2% 1-2 hours [56]
Toxocara NR 10 Centrifugal
flotation (3
different
methods: ([12,
60, 62])
Tween40
(0.5%)
NaNO3 (1.35) 46.83% <1 hour [55]
Tween80
(1%)
NaNO3 (1.35) 71% <1 hour
None ZnSO4 (1.2) 7.67% 3 hours
Toxocara Clay 210 Centrifugal
flotation X 3d,
recovery by
adherence to 4-5
coverslips
NaOH
(0.1 N)
ZnSO4 (1.18) 66.19% 1-2 hours [62]
Toxocara Silt 20 Centrifugal
flotation
Tween80
(0.1%)
NaCl (1.25) 45.3% 1-2 hours [63]
Sand NaCl (1.25) 100.8%
Laterite ZnSO4 (1.52) 56.8%
Toxocara NRd 0.48 Centrifugal
flotation X 4
Tween80
(0.0025%)
MgSO4 (1.275) 82.5% 1-2 hours [64]
Toxocara NR NR Centrifugal
flotation, recovery by
adherence to 2
coverslips
Tween80
(1%)
NaNO3 (1.35) 69.8% 1-2 hours [12]
Echinococcus NR 100 Centrifugal
flotation,
recovery by
adherence to
coverslip
Tween80
(0.05%)
Sucrose (1.27) 10% 2-3 hours [65]
Echinococcus Sand NR Centrifugal
flotation,
recovery by
adherence to
coverslip
Tween80
(0.05%)
ZnCl2 (1.4) NR 1-2 hours [66]
a

EPG - Eggs per gram.

b

Processing times estimated based on given times for incubation and centrifugation steps, and do not include time needed to examine the sample after recovery.

c

NR - not reported.

d

Multiple flotations performed (e.g. X 4 denotes four sequential flotations).