Table 3.

Reported sensitivities of genetic assays for STH detection and quantification.

Organism	Type	Life stage	Target gene(s)	Sample matrix	Extraction kit	Detection method	Limit of detectiona	Refs
Echinococcus multilocularis	PCR	Ova	12S rDNA	Soil	Sherlock AX Kit (A&A Biotechnology)	Electrophoresis	80% detection at 0.25 EPGb	[66]
Strongyloides stercoralis	LAMP	Larva	28S rRNA	Stool	Powersoil DNA Isolation Kit (Mobio)	Syto-82 fluorescent dye	0.4 larvae / mL	[87]
Taenia solium Taenia asiatica Taenia saginata	Multiplex PCR	Ova	Valine tRNA NADH dehydrogenase	Stool		NRc	10 EPG	[89]
Taenia solium Taenia asiatica Taenia saginata	LAMP	Ova	COX1 CLP	Stool	QIAamp DNA stool Minikit (QIAGEN)	Electro-phoresis	88.4% detection of single genome	[78]
Taenia spp	Multiplex PCR	Ova	COX1	Stool	QIAamp DNA Stool Minikit (QIAGEN)	Electro-phoresis	50 EPG	[90]
Toxocara canis	PCR	Ova (1 cell)	ITS-2 rDNA	Soil	NAd	Electro-phoresis	33% detection at 2 EPG	[82]
Ancylostoma caninum							0.4 EPG
Toxocara canis Toxocara cati	LAMP	Ova	ITS-2 rDNA	Sand	NA	Electro-phoresis	0.6 EPG	[79]
Strongyloides stercoralis	Multiplex quantitative PCR	Larvae	18S rRNA	Stool	QIAamp DNA Stool Minikit (QIAGEN)	Fluorescent Probes	0.4 larvae per gram	[91]
Trichuris spp	Quantitative PCR	Ova	ITS-1 rDNA	Stool	NA2	EvaGreen fluorescent dye	1 EPG3	[85]
Necator/ Ancylostoma spp			ITS-2 rDNA
Toxocara spp			23S rDNA
Ascaris lumbricoides	Quantitative PCR	Ova	ITS-1 rDNA	Sterile water	UltraClean microbial DNA and RNA kits (Mobio)	Fluorescent probes	3,750 copies or ~ 4 cells	[92]
Toxocara canis Toxocara Cati	Multiplex quantitative PCR	Ova	ITS-2 rRNA	Soil	PowerMax® soil DNA isolation kit (Mobio)	Fluorescent probes	2 EPG	[88]
Ancylostoma duodenale	Multiplex quantitative PCR	Ova	ITS-2 rDNA	Stool	QIAamp DNA Stool Mini kit (QIAGEN)	Fluorescent probes	1 copy per gram	[83, 93]
Necator americanus		Ova	ITS-2 rDNA				100 copies per gram
Ascaris lumbricoides		Ova	ITS-1 rDNA				1 copy per gram
Strongyloides stercoralis		Larvae	18S rDNA				1 copy per gram

^a Limits of detection are the lowest tested concentration at which 100% recovery was reported, unless otherwise stated.

^bEPG – eggs per gram.

^cNR – not reported.

^dNA – authors used their own in-house nucleic acid extraction techniques.