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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Cell Signal. 2015 Oct 28;28(1):91–99. doi: 10.1016/j.cellsig.2015.10.018

Figure 5. γ-Synuclein and PLCβ2 affect MDA MD 231 cell phenotypes.

Figure 5

Figure 5

A- Sample DIC images of MDA MB 231 cells mock treated or treated with γ-synuclein siRNA and compiled results showing a significant difference in the circularity of cells between the two groups (both n=360, p<0.001, Student’s t-test). B- Transwell migration assay showing the average number of cells treated with control siRNA, γ-synuclein and control siRNA, or γ-synuclein and PLCβ2 siRNA, that migrated per field, determined after fixing the transwell membrane and staining with DAPI after 4 hours of incubation (n=5, ANOVA). C- Transwell Matrigel invasion assay showing the average number of cells treated with control siRNA or γ-synuclein siRNA, that migrated per field, which was determined after fixing the transwell membrane and staining with DAPI after 4 hours of incubation (n=5, p<0.001 Student’s t-test). D- MTT assays were used to evaluate cell growth after treatment with control siRNA, γ-synuclein and control siRNA, or γ-synuclein and PLCβ2 siRNA. Data were expressed as a percentage of the absorbance of control cells at 570nm. The mean ± SEM is shown (n=6).