Recombinant selection by GST-LE. (A) GST-LE reacted with CK2 (adds 1 phosphate) was incubated with stoichiometric amounts of rCrm1 and rRan (+RCC1) at 300 mM or 500 mM NaCl. After glutathione bead extraction, bound proteins were visualized by Western analyses. (B) Similar to A, with no added NaCl, GST-LE without no phosphates (0), 1 added phosphate (1, CK2) or 2 added phosphates (2, CK2 plus Syk) was mixed with combinations of rCrm1, rRan and RCC1. Glutathione bead-extracted proteins were visualized by Western analyses. The bands were quantified by densitometry. For each lane, the ratio of Crm1 to GST signal was normalized to the 0 phosphate, no rRan sample (100%). This full experiment was carried out 4 times. The averaged values per sample type are indicated (SD ave 28)