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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Virology. 2015 Oct 19;487:19–26. doi: 10.1016/j.virol.2015.10.001

Figure 5.

Figure 5

Crm1 shRNA knockdown. (A) Plated HeLa cells transduced with an shRNA against human Crm1 were treated with doxycycline. After 0–4 days post-knockdown (PK) the cells were infected with vEC9 (MOI of 10). Lysates harvested 4 hr later were fractionated for Western analyses. Band intensities for 4 separate experiments, normalized to day 0 values and to the tubulin loading controls, are indicated. (B) Similar cells with (+) and without (−) induction were transfected (4 days PK) with LX-GST cDNAs. Harvest was at 16 hr post-transfection. Nup62 phosphorylation was determined as in A (n=2). (C) Infected cell lysates from A, after 0 days (−) and 4 days (+) PK were titered on normal HeLa cells. Plaque formation was evaluated after 28 hr. Observed titers (PFU/ml) are averaged from (n=3) replicate plates (SD: 0.02–0.28).