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. Author manuscript; available in PMC: 2016 Dec 10.
Published in final edited form as: Exp Cell Res. 2015 Nov 21;339(2):300–309. doi: 10.1016/j.yexcr.2015.11.021

Figure 1. Diagram of cTLR4 Construct and Engineered Receptor and Confirmation of cTLR4 construct expression.

Figure 1

(A) The cTLR4 construct contains: a myristolation domain (Myr), an engineered dimerization domain (F36V), the cytoplasmic portion of the TLR4 domain (cTLR4), a T2A ribosome skipping sequence, and a GFP tag. (B) Western blot probing for the FKBP12/F36V domain (35.5 kDa) containing lysates from RAW264.7 cells, MΦ-T2A negative control cells, MΦ-cTLR4 cells, and F2 positive control cells. MΦ-T2A cells have been transduced with a construct that contains only the T2A ribosome skipping sequence with the GFP tag. F2 cells have been transduced with just two adjacent F36V domains.