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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Virology. 2015 Oct 27;487:112–120. doi: 10.1016/j.virol.2015.10.003

Figure 5. Interaction of LPV products with the RB/E2F pathway.

Figure 5

A) Western blot analysis of p130 and p107 proteins in MEFs expressing different LPV products. SV40.ER-expressing MEFs were used as positive control. GAPDH was used as loading control. B) Real-time PCR analysis for canonical E2F target genes using gene specific primers. The gene expression levels are represented relative to the corresponding expression levels in wt MEFs, and Rpl5 expression levels were used for cDNA normalization. The error bars represent standard deviation; within each sample, each gene was analyzed in quadruplicate.