Fig. 6.

Dissemination of reovirus T3D to the CNS is limited by type I IFN responses and older age but not inefficient retrograde axonal transport. IFNAR+/+ and IFNAR−/− mice were inoculated intramuscularly with 10⁷ PFU total of nine genetically marked reoviruses, with or without additional muscle damage. Viral titers were determined by plaque assay, and viral population diversity was determined using a hybridization-based assay. Reovirus T3D titer (A) and viral population diversity (B) in tissues harvested from adult IFNAR+/+ or IFNAR−/− mice with or without muscle damage. Tissues were collected at 72 hpi. (C) Reovirus T3D titer in tissues from adult mice collected at 7 d post-inoculation. Reovirus T3D titer (D) and viral population diversity (E) in tissues from 3-day-old IFNAR+/+ or IFNAR−/− mice. Tissues were collected at 72 hpi, prior to disease onset. Results are presented as mean +/− standard error of the mean from 5-9 mice per condition. Values that are significantly different, as determined by the Mann-Whitney test, are indicated by asterisks as follows: *, P < 0.05, **, P < 0.005, ***, P < 0.0005. Mus., Muscle, SN, Sciatic Nerve, SC, Spinal Cord, Br., Brain. ND, none detected.