Fig. 7.

Co-infection with poliovirus does not enhance reovirus dissemination to the CNS. IFNAR+/+ and IFNAR−/− mice were inoculated intramuscularly with 10⁷ PFU total of nine genetically marked reoviruses and 10⁷ PFU total of 10 genetically marked polioviruses. Tissues were collected at 72 hpi. Reovirus titers and population diversity were determined by plaque assay and hybridization-based assay, respectively, using L929 cells, which do not support poliovirus replication. Reovirus T3D titer (A) and viral population diversity (B) in tissues harvested from adult IFNAR+/+ or IFNAR−/− mice with or without muscle damage. ‘ind’ indicates data from infections with reovirus only (from Fig. 6A-B data), and ‘co’ indicates data from the reovirus-poliovirus co-infection. Results are presented as mean +/− standard error of the mean from 5-7 mice per condition. Values that are significantly different, as determined by the Mann-Whitney test, are indicated by asterisks as follows: *, P < 0.05. ND, none detected.