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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Exp Mol Pathol. 2015 Aug 29;99(3):455–459. doi: 10.1016/j.yexmp.2015.08.011

Figure 1. Targeting scheme for and verification of deletion of CD99L2 by homologous recombination.

Figure 1

See Materials and Methods for details. A. Two forward primers and a common reverse primer were designed for a multiplex PCR system. Black boxes with numbers denote exons. PGK-NEO cassette in the inverse orientation is shown in the knockout allele. B. PCR results from wild-type, heterozygous, and CD99L2-deficient mice. Numbers at the right indicate the molecular size in base pairs.