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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Ann N Y Acad Sci. 2015 Aug 20;1362(1):77–85. doi: 10.1111/nyas.12865

Figure 4.

Figure 4

Peritoneal IL-10 gene expression is significantly reduced upon L2pB1 cell depletion. Single-cell suspension was obtained from PCW a day after four consecutive intraperitoneal injections of PBS or DT (one injection per day). RNA was extracted, followed by reverse transcription and rea-time PCR. Relative gene expression levels of IL-10 and CD33 of PBS control (n = 3; dark bar) and DT-treated mice (n = 3; light bar) are normalized against expression of the housekeeping protein GAPDH. Triplicate PCR was performed for each gene from each sample, and the average gene expression level relative to GAPDH is shown with standard deviation. Student's t-test was performed and P values < 0.05 are shown.