Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Cell Signal. 2015 Oct 19;28(1):43–52. doi: 10.1016/j.cellsig.2015.10.004

Figure 1. Mitotic-dependent phosphorylation of LARG.

Figure 1

(A) Mitotic-dependent mobility shift of LARG. HeLa cells were treated with nocodazole as described in Materials and Methods. Cells arrested in prometaphase (M) were collected by mitotic shake-off, and cells remaining on plates were collected and designated as G2 cells. Lysates from asynchronous (A), G2, or prometaphase-arrested (M) HeLa cells were immunoblotted with anti-LARG antibody. (B) Phosphatase treatment reverses mitotic-dependent phosphorylation of LARG. G2 or prometaphase-arrested (M) lysates from HeLa (left panels) and COS-7 (right panels) cells were prepared as above in A. Lysates from prometaphase-arrested (M) cells were then treated with or without λ protein phosphatase, followed by immunoblotting with anti-LARG antibody or anti-GAPDH antibody (loading control).