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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Eur J Pharmacol. 2015 Nov 6;769:134–142. doi: 10.1016/j.ejphar.2015.11.008

Fig. 2.

Fig. 2

Dasatinib inhibits αSMA synthesis, Src pY416 phosphorylation in NIH 3T3 fibroblasts. A. Western blot images of NIH 3T3 lysates treated in the presence and absence of 100 pM TGFβ (48 h) and TGFβ (48 h total) in combination with 10 nM dasatinib or 10 µM PP2 (24 h), probed αSMA, SRF, pY416Src and total Src. B. Densitometry analysis of the Western bands showing expression changes in αSMA and SRF with 100 pM TGFβ (48 h) alone and in combination with 10 nM dasatinib or 10 µM PP2 (24 h), and normalized to GAPDH. (n=3). C. Densitometry analysis of the Western bands showing expression changes in pY416Src and total Src with 100 pM TGFβ (48 h) alone and in combination with 10 nM dasatinib or 10 µM PP2 (24 h), normalized to GAPDH (n=3). Data presented as mean ± S.D. *P<0.05; #P<0.01; §P<0.001