Fig. 3.

Dasatinib inhibits TGFβ-induced αSMA expression and fibronectin assembly in NIH3T3 fibroblasts. A. Representative images of NIH3T3 fibroblast layers subjected to serum starvation followed by treatment with 100 pM TGFβ (48 h) and in combination with 10 nM dasatinib or 10 µM PP2 (24 h), followed by probing with anti-αSMA and anti-fibronectin primary antibodies as well as Alxa Flour 488-labeled secondary antibodies. B. Bar graph showing NIH-Image J based quantitative analysis of assembled fibronectin by NIH 3T3 fibroblasts in the presence or absence of TGFβ and co-treatment with 10 nM dasatinib or 10 µM PP2 in serum free medium (n=3). Data presented as mean ± S.D. Scale bar: 50 µm. *P<0.05; #P<0.01; §P<0.001