. 2015 Oct 16;21(1):167–178. doi: 10.1007/s12192-015-0650-8

Table 1.

Primer pairs for real-time PCR

Gene	Primer	Product length (bp)
HspB1 (Hsp25)	Fwd: CGGCAACTCAGCAGCGGTGTCT	160
HspB1 (Hsp25)	Rev: CATGTTCATCCTGCCTTTCTTCGTG	160
HspB2 (MKBP)	Fwd: CCGAGTACGAATTTGCCAACCC	191
HspB2 (MKBP)	Rev: AAATGCCTGGAACTTGCCTTCACT	191
HspB3	Fwd: AACCAGAATGGACGAACACGG	104
HspB3	Rev: CATGACAGAGGATGGCAGACAAAT	104
HspB4 (αA-crystallin)	Fwd: AGATGGCTGCGGGTTCA	154
HspB4 (αA-crystallin)	Rev: GGCTCTTGGGGTTGGTTAG	154
HspB5 (αB-crystallin)	Fwd: CTTCTACCTTCGGCCACCCTC	164
HspB5 (αB-crystallin)	Rev: GCACCTCAATCACGTCTCCC	164
HspB6 (Hsp20)	Fwd: CCATGTGGAGGTCCATGCTCG	195
HspB6 (Hsp20)	Rev: GCAGGTGGTGACGGAAGTGAG	195
HspB7 (cvHsp)	Fwd: CCACCTCCAACAACCACATCG	100
HspB7 (cvHsp)	Rev: ACATCCTCTGGTAGCTGGCACTT	100
HspB8 (Hsp22)	Fwd: CCGGAAGAACTGATGGTAAAGAC	166
HspB8 (Hsp22)	Rev: CCTCTGGAGAAAGTGAGGCAAATAC	166
HspB9	Fwd: GCCCTTTCTGAACGAAATCAAGC	159
HspB9	Rev: GCCATCTATCCGCACCACCA	159
HspB10 (ODF1)	Fwd: AACGTCTGCGGCTTTGAACCT	195
HspB10 (ODF1)	Rev: ACTGCCGAGCCCGTAGGAGTAGGTC	195
HspB11 (Hsp16.2)	Fwd: ATTATGGCACGAGATGGCTACG	128
HspB11 (Hsp16.2)	Rev: TGTAATCACTAAGGAAGACTTGAGACTG	128
Hsp70i	Fwd: GGTCATCTCCTGGCTGGACTCTAACACG	212
Hsp70i	Rev: GCCAGAAAAGCCTCTAATCCACCTCC	212
ACTB	Fwd: TCCGTAAAGACCTCTATGCCAACA	104
ACTB	Rev: GCTAGGAGCCAGGGCAGTAATCT	104
Ywhaz	Fwd: TTGAGCAGAAGACGGAAGGT	136
Ywhaz	Rev: GAAGCATTGGGGATCAAGAA	136

Hsp70i inducible form of Hsp70, ACTB beta-actin, Ywhaz tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta