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. 2015 Sep 22;91:56–67. doi: 10.1007/s12565-015-0300-3

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — High-magnification images showing the expression of β-galactosidase in the thalamic nuclei (a, c, e, g) and laterodorsal tegmental nuclei (b, d, f, h) of B6.Cg-Tg(Nes-cre)1Nogu mouse brain, revealed by a combination of X-gal (a, b, e, f) or Salmon-gal (c, d, g, h) with potassium ferri- and ferro-cyanide (a, b, c, d) or nitroblue tetrazolium (e, f, g, h). Reaction time is 24 h for the X-gal/nitroblue tetrazolium, X-gal or Salmon-gal with potassium ferri- and ferro-cyanide staining mixtures, and 5 h for Salmon-gal/nitroblue tetrazolium. Scale bar 300 µm (a, c, e, f) and 200 µm (b, d, g, h)