Skip to main content
. 2015 Dec 16;35(50):16362–16376. doi: 10.1523/JNEUROSCI.1392-15.2015

Figure 6.

Figure 6.

Dnmt3a knock-out from NAc in females creates a hybrid male/female transcriptional state in this brain region. a, Heatmap of genes differentially expressed in Dnmt3a knock-out females compared with GFP males that underwent SCVS. b, Comparison of genes upregulated in males and Dnmt3a knock-out females. c, Comparison of genes downregulated in males and Dnmt3a knock-out females. d, Comparison of the percentage of genes that overlap in SCVS males and females with the overlap in SCVS males and Dnmt3a knock-out females. e, Heatmap of genes differentially regulated by SCVS females compared with Dnmt3a knock-out females. Dnmt3a knock-out neutralizes a subset of genes regulated by stress in females. f, Canonical pathways altered by SCVS in females compared with the same pathways in Dnmt3a knock-out SCVS females and stressed males. g, Validation by qRT-PCR of a subset of genes regulated by SCVS in females from the CRF pathway (n = 9–17/gene/condition). h, Genes regulated by SCVS in the CRF pathway are neutralized by removing Dnmt3a (n = 5–8/gene/condition). i, Network analysis of Dnmt3a knock-out females. Libraries were created from pooled mRNA samples (n = 4 mice/library and run in independent biological replicates of 3 libraries per sex/stress). *Significant difference in means (unpaired t test, two tailed).