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. 2015 Dec 16;35(50):16377–16397. doi: 10.1523/JNEUROSCI.3237-15.2015

Table 1.

Composition of buffers used in pH imaging experiments^a

	NaHCO₃ buffer				KHCO₃ buffer				HCO₃⁻-free buffer
	Normal	NH₄⁺	Cl⁻-free		Normal	NH₄⁺	Na⁺-free		Normal	NH₄⁺	Na⁺-free
	Normal	NH₄⁺	Normal	NH₄⁺	Normal	NH₄⁺	Normal	NH₄⁺	Normal	NH₄⁺	Normal	NH₄⁺
NaCl	125	105			130	110			145	125
KCl	5	5							5	5	5	5
MgCl₂	5	5			5	5	5	5	5	5	5	5
CaCl₂	1	1			1	1	1	1	1	1	1	1
Glucose	20	20	20	20	20	20	20	20	20	20	20	20
HEPES	10	10	10	10	10	10	10	10	10	10	10	10
NaHCO₃	20	20	20
KHCO₃					20	20	20	20
NH₄Cl		20				20		20		20		20
NMDG							130	110			145	125
Na-gluconate			125	125
K-gluconate			5	5
Mg-gluconate			5	5
Ca-gluconate			1	1
NH₄HCO₃				20

^aAll numbers are in mm concentration.