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. 2015 Dec 16;35(50):16494–16503. doi: 10.1523/JNEUROSCI.1691-15.2015

Figure 7.

Figure 7.

The increase in calcium transients elicited by the D1R agonist SKF-38393 is dependent upon Cav1.3a activity. Calcium transients were ratiometrically imaged in the hair cells of Tg(myo6b:D3) larvae. Hair cells were mechanically stimulated with a 2 s step stimulus. A, Averaged traces with shaded SE for DMSO control (blue), 100 nm SKF-38393 treated (purple), and agonist treated and washed (green) hair cells. Duration of the 2 s stimulus is indicated by gray area. B, Data from 19 individual hair cells. C, Averaged traces with shaded SE for DMSO control (blue), 10 μm isradipine treated (mint), and isradipine pretreated cells exposed to 100 nm SKF-38393 (purple). D, Data from 9 individual hair cells. E, Mean amplitudes (Maximum FRET Ratio) of evoked calcium transients in control and drug-treated hair cells. Con versus SKF, p = 0.0011; Con versus SCH, p = 0.0058; ISR versus ISR + SKF, p = 0.18. ** p < 0.01; ns, Not significant.