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. 2015 Dec 16;6:1122. doi: 10.3389/fpls.2015.01122

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Copyright © 2015 Wu, Wu, Yin, Zheng and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sphingolipid profiles of wild-type, ein2, and ctr1-1 seedlings during FB1 treatment. Seven-day-old seedlings were treated with 0.5 μM FB1 and collected after 6 days. Total sphingolipids were extracted and analyzed by LC–MS system. (A) Phenotypes of FB1-treated seedlings. Photos were taken after 6 days of treatment. (B) Quantitation of the phenotypes in (A). Seedlings with lesions were scored as having a severe phenotype. The data represent at least three independent experiments. More than 100 seedlings were counted. (C–E) Comparisons of different kinds of sphingolipids in wild-type, ein2, and ctr1-1 seedlings. Data represent means ± SD from three independent biological experiments and sets marked with different letters indicate significances assessed by Student–Newman–Keul test (P < 0.05). (C) LCBs. (D) Ceramides. (E) Hydroxyceramides. See Supplementary Figure S1 for the detailed analysis.