FIGURE 6.

Actively vaccinated mice and sham vaccinated mice were challenged with Y. enterocolitica 8081 to assess the memory immune protection. (A) Group of BALB/c mice scheduled immunized for 42nd day with 50 μg of recombinant proteins (rV, rE, and rVE) and left uninfected for 120 days. After incubation of 120 days mice were IP injected with 10⁸ CFU of Y. enterocolitica and survival was monitored for 24 days. Sham immunized mice were considered as control. Data represented as the percentage survival calculated by Kaplan–Meier method using Graphpad Prism5. (B) Bacterial burden in immunized and sham immunized mice challenged with Y. enterocolitica 8081. The analysis of total Colony Forming Units (CFU) in liver (i), spleen (ii), were performed at different time points (day 2, 7, 14, and 24) post challenge. Data pooled from 2 to 4 biological replicates in two statistical repeats were analyzed by Univariate method applying Dunnett’s and Tukey’stest. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.