Table 1.

atnp01 and atnp02 proteins influenced by mutations and by exposure to 80 mg L⁻¹ CdS QDs, separated by 2D-LC and identified by MALDI-TOF/MS and in silico analysis.

Frac.a	RTb	Protein name (UniProt database)c	Accession no.d	Genee	Locusf	Probeg	Massh	pIi	Scorej	Expectk	Matchl	Cov.m (%)
14	20.15	12S seed storage protein CRC	CRU3_ARATH	CRU3	At4g28520	253767_AT	58,235	6.99	54	0.047	18	23
27	24.50	14-3-3-like protein GF14 nu	14337_ARATH	GRF7	At3g02520	258489_AT	29,920	4.74	39	1.5	4	18
17	19.32	2S seed storage protein 1	2SS1_ARATH	AT2S1	At4g27140	253904_AT	19,013	5.70	50	0.16	9	31
11	20.00	2S seed storage protein 3	2SS3_ARATH	AT2S3	At4g27160	253895_AT	18,762	7.86	51	0.11	11	38
24	14.91	4-alpha-glucanotransferase DPE2	DPE2_ARATH	DPE2	At2g40840	245094_AT	110,562	5.54	49	0.18	14	14
7	20.18	Actin-2	ACT2_ARATH	ACT2	At3g18780	257749_AT	42,078	5.37	62	0.008	11	37
29	34.09	Alanine–tRNA ligase	SYA_ARATH	ALATS	At1g50200	262468_AT	111,275	6.05	45	0.38	13	21
13	20.43	Arogenate dehydratase/prephenate dehydratase 6	AROD6_ARATH	ADT6	At1g08250	261758_AT	45,059	6.11	41	0.96	5	28
8	24.56	ATP synthase subunit beta, chloroplastic	ATPB_ARATH	atpB	AtCg00480	245014_AT	53,957	5.38	45	0.38	7	19
28	26.67	ATP-dependent DNA helicase Q-like 4A	RQL4A_ARATH	RECQL4A	At1g10930	17661_AT	134,654	6.82	45	0.39	12	14
22	16.14	Auxilin-like protein 1	AUL1_ARATH	AUL1	At1g75310	261117_AT	163,087	4.92	60	0.016	37	22
8	23.62	Auxin transport protein BIG	BIG_ARATH	BIG	At3g02260	259128_AT	574,541	5.65	54	0.047	30	7
15	19.30	Beta-amylase 3, chloroplastic	BAM3_ARATH	BAM3	At4g17090	245346_AT	61,713	6.59	40	1.2	20	41
8	26.25	BTB/POZ domain-containing protein At5g67385	Y5738_ARATH	At5g67385	At5g67385	–	68,086	8.16	34	36	3	4
27	18.30	Calcium-binding protein CML31 (probable)	CML31_ARATH	CML31	At2g36180	263903_AT	16,375	4.21	47	0.25	7	53
14	13.37	Calcium-binding protein CML42	CML42_ARATH	CML42	At4g20780	254487_AT	21,191	4.59	55	0.043	7	34
21	17.08	Calcium-binding protein CML45 (Probable)	CML45_ARATH	CML45	At5g39670	249417_AT	24,104	4.93	33	6.9	5	34
4	17.01	Calcium-dependent protein kinase 23	CDPKN_ARATH	CPK23	At4g04740	255306_AT	59,073	6.09	60	0.001	11	23
22	14.95	Calmodulin-like protein 1	CML1_ARATH	CML1	At2g15680	265494_AT	16,3087	4.92	46	0.34	31	18
29	34.09	CLIP-associated protein	CLASP_ARATH	CLASP	At2g20190	265315_AT	160,107	6.72	46	0.34	31	22
2	22.23	Cyclic nucleotide-gated ion channel 6 (Probable)	CNGC6_ARATH	CNGC6	At2g23980	266520_AT	86,299	9.38	45	0.42	12	19
24	15.87	Defensin-like protein 121 (Putative)	DF121_ARATH	LCR55	At3g20997	–	8969	7.51	42	0.85	5	67
14	19.90	Defensin-like protein 192	DF192_ARATH	ATTI7	At1g47540	262431_AT	11,063	6.34	44	0.45	8	55
28	21.11	Defensin-like protein 37	DEF37_ARATH	EDA21	At4g13235	–	9708	9.3	40	1.2	3	36
2	24.91	Defensin-like protein 90	DEF90_ARATH	At1g54445	At1g54445	–	9175	8.44	40	1.4	3	57
18	15.50	DNA damage-binding protein 1b	DDB1B_ARATH	DDB1B	At4g21100	254452_AT	121,178	4.97	44	0.45	6	44
26	23.93	E3 ubiquitin-protein ligase ARI9 (probable)	ARI9_ARATH	ARI9	At2g31770	263463_AT	64,529	4.96	45	0.44	11	19
24	17.90	ELM2 domain-containing protein	D7KQK8_ARAL	At1g13880	At1g13880	259423_AT	48,428	4.18	50	0.15	14	40
14	21.12	F-box/kelch-repeat protein At1g48625	FBK20_ARATH	At1g48625	At1g48625	–	48,704	8.9	51	0.11	11	32
29	34.12	F-box/kelch-repeat protein At3g43710 (putative)	FBK72_ARATH	At3g43710	At3g43710	–	44,064	7.91	40	1.3	8	15
5	23.07	Galacturonosyltransferase 2 (putative)	GAUT2_ARATH	GAUT2	At2g46480	265477_AT	62,393	6.98	34	5.2	4	16
3	25.23	GDP-mannose 4,6 dehydratase 1	GMD1_ARATH	GMD1	At5g66280	247094_AT	40,939	6.61	41	0.94	6	26
23	20.37	GDP-mannose 4,6 dehydratase 2	GMD2_ARATH	MUR1	At3g51160	252121_AT	40,939	6.61	53	0.07	5	22
22	15.87	GDSL esterase/lipase ESM1	ESM1_ARATH	ESM1	At3g14210	257008_AT	44,374	7.59	44	0.49	6	18
19	16.55	Glucan endo-1,3-beta-glucosidase	E13A_ARATH	BGL2	At3g57260	251625_AT	37,338	4.60	44	0.60	9	25
10	23.05	Glutaredoxin-C14	GRC14_ARATH	GRXC14	At3g62960	251197_AT	11,561	7.66	44	0.45	4	66
12	17.40	Glutathione S-transferase DHAR3	DHAR3_ARATH	DHAR3	At5g16710	246454_AT	28,724	7.59	35	4.3	12	41
14	18.51	Glutathione S-transferase U24	GSTUO_ARATH	GSTU24	At1g17170	262518_AT	25,461	5.85	56	0.031	17	47
22	14.51	Glycine cleavage system H protein 2	GCSH2_ARATH	GDH2	At2g35120	266517_AT	17,203	4.97	49	0.18	8	31
27	22.01	Heat shock 70 kDa protein 10, mitochondrial	HSP7J_ARATH	HSP70-10	At5g09590	250502_AT	73,174	3.5	36	3.5	5	9
28	27.65	Homeobox-leucine zipper protein ATHB-7	ATHB7_ARATH	ATHB-7	At2g46680	266327_AT	30,663	5.52	42	0.9	12	50
24	18.36	LOB domain-containing protein 5	LBD5_ARATH	LBD5	At1g36000	260187_AT	14,477	6.73	34	4.9	8	44
19	19.62	Lysine-specific histone demethylase 1	LDL1_ARATH	LDL1	At1g62830	262668_AT	93,767	4.84	64	0.005	10	17
26	24.89	Mechanosensitive ion channel protein 6	MSL6_ARATH	MSL6	At1g78610	263127_AT	97,057	8.73	42	0.8	13	16
22	15.98	Methyl-CpG-binding domain-containing protein 9	MBD9_ARATH	MBD9	At3g01460	258950_AT	243,929	5.34	54	0.059	32	17
2	24.12	N-(5'-phosphoribosyl)anthranilate isomerase 1	PAI1_ARATH	PAI1	At1g07780	259770_S_AT	29,847	8.63	35	3.7	5	32
28	24.68	NF-X1-type zinc finger protein NFXL2	NFXL2_ARATH	NFXL2	At5g05660	250767_AT	105,547	8.63	43	0.67	14	18
27	24.20	Nudix hydrolase 21	NUD21_ARATH	NUDT21	At1g73540	245777_AT	22,793	8.39	52	0.084	4	43
2	21.30	Oleosin GRP-17	GRP17_ARATH	GRP17	At5g07530	250637_AT	53,330	10.34	36	3.5	15	36
4	18.20	Pathogenesis-related protein 1	PR1_ARATH	At2g14610	At2g14610	266385_AT	17,676	8.96	48	0.20	8	35
18	17.80	Pathogenesis-related protein 5	PR5_ARATH	At1g75040	At1g75040	259925_AT	25,252	4.54	53	0.040	16	43
27	23.98	Pectate lyase 9 (probable)	PLY9_ARATH	At3g24230	At3g24230	257243_AT	50,638	8.65	48	0.22	11	31
28	19.63	Pentatricopeptide repeat-containing protein At1g12775	PPR39_ARATH	At1g12775	At1g12775	261194_AT	73,474	6.15	44	0.46	10	28
2	24.33	Pentatricopeptide repeat-containing protein At1g62590	PPR90_ARATH	At1g62590	At1g62590	265106_S_AT	71,899	7.97	53	0.061	6	9
24	15.36	Pentatricopeptide repeat-containing protein At1g62670	PPR91_ARATH	At1g62670	At1g62670	260792_AT	110,562	5.54	50	0.15	10	10
27	19.43	Pentatricopeptide repeat-containing protein At1g63330	PP101_ARATH	At1g63330	At1g63330	265106_S_AT	63,778	6.83	36	2.9	6	10
27	21.94	Pentatricopeptide repeat-containing protein At2g18940	PP163_ARATH	At2g18940	At2g18940	266951_AT	93,433	8.36	36	3.6	13	20
14	24.38	Pentatricopeptide repeat-containing protein At2g45350	PP202_ARATH	CRR4	At2g45350	245129_AT	70,052	6.78	46	0.37	5	10
27	21.44	Pentatricopeptide repeat-containing protein At3g29290	PP262_ARATH	EMB2076	At3g29290	256613_AT	63,160	9.01	38	2.1	5	12
24	27.45	Pentatricopeptide repeat-containing protein At3g57430	PP285_ARATH	PCMP-H81	At3g57430	251631_AT	100,466	6.94	37	2.7	15	15
26	25.63	Pentatricopeptide repeat-containing protein At4g26680	PP338_ARATH	At4g26680	At4g26680	253979_AT	60,010	9.45	47	0.28	12	28
24	13.03	Pentatricopeptide repeat-containing protein At5g61400	PP440_ARATH	At5g61400	At5g61400	247548_AT	75,760	7.91	37	2.5	10	14
26	26.77	Peptide methionine sulfoxide reductase B7	MSRB7_ARATH	MSRB7	At4g21830	254385_S_AT	15,789	8.54	51	0.11	4	36
4	14.49	Phosphomethylpyrimidine synthase	THIC_ARATH	THIC	At2g29630	266673_AT	72,568	6.00	46	0.31	9	22
26	26.02	Prefoldin subunit 4 (probable)	PFD4_ARATH	AIP3	At1g08780	264778_AT	14,985	4.55	41	1	5	54
14	13.68	Pre-mRNA-splicing factor SLU7-A	SLU7A_ARATH	At1g65660	At1g65660	264633_AT	62,281	5.73	41	1.1	12	21
4	11.96	Probable beta-D-xylosidase 7	BXL7_ARATH	BXL7	At1g78060	262181_AT	84,751	8.3	66	0.003	15	22
1	12.50	Proline-rich extensin-like protein EPR1	EPR1_ARATH	EPR1	At2g27380	265644_AT	81,946	10.61	60	0.009	12	19
29	28.05	Prolyl 4-hydroxylase 7 (probable)	P4H7_ARATH	P4H7	At3g28480	257844_AT	36,117	6.32	43	0.64	7	20
16	18.93	Proteasome subunit alpha type-1-A	PSA1A_ARATH	PAF1	At5g42790	249161_AT	30,685	4.99	45	0.4	7	27
29	34.48	Proteasome subunit alpha type-3	PSA3_ARATH	PAG1	At2g27020	266312_AT	27,645	5.93	62	0.008	9	24
19	21.88	Protein FLX-like 2	FLXL2_ARATH	FLXL2	At1g67170	–	39,831	6.93	48	0.21	7	23
15	19.32	Protein KTI12 homolog	KTI12_ARATH	DLR1	At1g13870	259450_AT	34,063	5.97	44	0.73	9	33
29	27.96	Protein MODIFIER OF SNC1 1	MOS1_ARATH	MOS1	At4g24680	254143_AT	153,766	9.39	53	0.067	21	19
7	20.45	Protein phosphatase 2C 13 (probable)	P2C13_ARATH	At1g48040	At1g48040	260722_AT	42,649	4.85	52	0.088	10	25
24	19.84	Protein TONNEAU 1b	TON1B_ARATH	TON1B	At3g55005	251816_AT	29,338	5.06	37	3	14	46
22	17.02	Protein VERNALIZATION INSENSITIVE 3	VIN3_ARATH	VIN3	At5g57380	–	71,128	5.88	39	1.6	16	26
27	31.71	Proton pump-interactor 3A	PPI3A_ARATH	PPI3A	At5g36780	–	66,923	6.07	33	6.2	7	14
2	24.88	Ribulose bisphosphate carboxylase large chain	RBL_ARATH	rbcL	AtCg00490	245015_AT	53,435	5.88	49	0.16	10	23
2	22.76	Ribulose bisphosphate carboxylase small chain 1A	RBS1A_ARATH	RBCS-1A	At1g67090	264474_S_AT	20,488	7.59	101	1.6e-07	12	68
3	22.72	Ribulose bisphosphate carboxylase small chain 2B	RBS2B_ARATH	RBCS-2B	At5g38420	264474_S_AT	20,622	7.59	101	0.00001	13	59
3	23.95	Ribulose bisphosphate carboxylase small chain 3B	RBS3B_ARATH	RBCS-3B	At5g38410	264474_S_AT	20,556	8.22	54	0.048	8	53
26	25.17	RING-H2 finger protein ATL38	ATL38_ARATH	ATL38	At2g34990	267417_AT	34,890	6.71	43	0.64	8	36
3	24.77	Rop guanine nucleotide exchange factor 1	ROGF1_ARATH	ROPGEF1	At4g38430	252975_S_AT	61,380	5.44	40	1.3	8	20
26	26.52	Transcription factor GTE1	GTE1_ARATH	GTE1	At2g34900	257352_AT	43,586	6.04	45	0.37	7	26
26	25.09	WPP domain-interacting tail-anchored protein 1	WIT1_ARATH	WIT1	At5g11390	250363_AT	79,228	4.7	39	1.6	12	13
12	21.17	WRKY transcription factor 61 (probable)	WRK61_ARATH	WRKY61	At1g18860	261429_AT	53,224	6.48	35	4.1	10	18
12	17.80	γ-interferon responsive lysosomal thiol (GILT) reductase family protein	F4JRI7_ARATH	At4g12960	At4g12960	254777_AT	27,196	7.05	38	3.7	14	44

^afraction,

^bprogressive peak number as given by 2D-LC,

^cputative protein identification,

^daccession number for the closest match in the UniProt database,

^egene,

^flocus,

^gprobe,

^hpredicted mass value,

ⁱpI of the closest match in the database,

^jscore,

^Kexpected value of the database research,

^lmatches,

^mpercentage of coverage of the matching peptide sequence tags derived by applying MASCOT algorithm.