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. 2015 Dec 16;6:1094. doi: 10.3389/fpls.2015.01094

Figure 1.

Figure 1

(A) Schematic representation of OsXPB2 promoter cloned in pCAMBIA1391Z vector (promoter less vector) at PstI and BamHI sites for measuring GUS activity and agro-infiltration. (B–J) time course of OsXPB2 promoter-GUS expression analysis in the agro-infiltrated tobacco leaves in response to abiotic stress [200 mM salt, 20% PEG, cold (4°C) stress] and phytohormones [MeJA (10 μM), ABA (5 μM), and auxin (10 μM)]. Histochemical GUS staining of OsXPB2 promoter::GUS treated with water (mock treated) (B), CaMV35S::GUS water (mock treated) (C), OsXPB2::GUS NaCl (D), PEG (E), cold (F), Auxin (G), MeJA (H), ABA (I), comparison of GUS activity determined in protein extracts (in vitro) (J). Data of four independent agro-infiltrated leaves were measured, and each experiment was replicated four times. Error bars on the graphic represent (±SD). *P < 0.05 differ significantly from their respective controls according to Student's paired t-test.