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. 2015 Dec 16;6:1369. doi: 10.3389/fmicb.2015.01369

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Copyright © 2015 Grande, Di Marcantonio, Robuffo, Pompilio, Celia, Di Marzio, Paolino, Codagnone, Muraro, Stoodley, Hall-Stoodley and Mincione.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DNase I-gold complexes labeling of 2-days old H. pylori ATCC 43629/NCTC 11639 mature biofilms. The colloidal gold labeled DNase I particles are used to detect the eDNA. The arrow represents the presence of eDNA on bOMVs membrane surface (b,B,C,E); while the arrow head represents the presence of eDNA on bacteria cell walls (B). OMVs are present between adjacent bacterial cells (circles, a,B,C). Representative arrangements between cells and vesicles are shown at higher resolution by the dashed squares “a” and “b” in (A). Panel (D) shows a single vesicle labeled with colloidal gold labeled DNase I particles.