Table 1.
Primer sequences for real-time polymerase chain reaction (PCR) quantification
| Gene | Primer pair sequences | Amplicon size |
|---|---|---|
| HPRT F | 5'-CCTGGCGTCGTGATTAGTGAT-3' | 131 bp |
| HPRT R | 5'-AGACGTTCAGTCCTGTCCATAA-3' | |
| TNF-α F | 5´-CCCAGGCAGTCAGATCATCTTC-3´ | 85 bp |
| TNF-α R | 5´-AGCTGCCCCTCAGCTTGA-3´ | |
| IL-1β F | 5'-ATGGCTTATTACAGTGGCAATGAG-3' | 138 bp |
| IL-1β R | 5'-GTAGTGGTGG TCGGAGATTCG-3' | |
| INF-γ F | 5'-AGCGGATAATGGAACTCTTTTCTTAG-3' | 103 bp |
| INF-γ R | 5'-AAGTTTGAAGTAAAAGGAGACAATTTGG-3' |
HPRT: Hypoxanthine-guanine phosphoribosyltransferase; TNF-α: Tumor necrosis factor-alpha; IL-1β: Interleukin-1 beta; IFN-γ: Interferon-gamma