Table 3.
(A) Association between respirable dust (measured and calculated) exposure and CpG sites methylation in F2RL3 in welders and (B) linear regression analysis of working years as welder and F2RL3 methylation
| Panel A | ||||||||
|---|---|---|---|---|---|---|---|---|
| Variable | Measured respirable dust (N=53) | Calculated respirable dust (N=99) | ||||||
| Unadjusted analysis* | Full model† | Unadjusted analysis* | Full model‡ | |||||
| β (95% CI) | p Value | β (95% CI) | p Value | β (95% CI) | p Value | β (95% CI) | p Value | |
| CpG_2 | −0.51 (−0.85 to −0.16) | 0.0045 | −0.49 (−0.88 to −0.10) | 0.014 | −0.44 (−0.85 to −0.036) | 0.033 | −0.38 (−0.79 to 0.025) | 0.065 |
| CpG_3 | −0.12 (−0.31 to 0.076) | 0.23 | −0.059 (−0.28 to 0.16) | 0.59 | −0.19 (−0.39 to 0.0057) | 0.057 | −0.17 (−0.38 to 0.039) | 0.11 |
| CpG_4 | −1.4 (−2.0 to −0.80) | <0.001 | −1.1 (−1.7 to −0.46) | 0.0011 | −1.0 (−1.7 to −0.35) | 0.0032 | −0.71 (−1.4 to −0.062) | 0.032 |
| CpG_5 | −0.64 (−1.1 to −0.15) | 0.012 | −0.64 (−1.2 to −0.069) | 0.029 | −0.25 (−0.81 to 0.32) | 0.39 | −0.089 (−0.66 to 0.48) | 0.76 |
| Panel B: Working years as welder (N=100) | ||||||
|---|---|---|---|---|---|---|
| Variable | Unadjusted analysis* | Adjusted analysis§ | Full model¶ | |||
| β (95% CI) | p Value | β (95% CI) | p Value | β (95% CI) | p Value | |
| CpG_2 | −0.027 (−0.091 to 0.037) | 0.41 | −0.019 (−0.082 to 0.044) | 0.56 | −0.011 (−0.075 to 0.053) | 0.73 |
| CpG_3 | −0.020 (−0.051 to 0.011) | 0.20 | −0.020 (−0.051 to 0.011) | 0.21 | −0.018 (−0.051 to 0.015) | 0.28 |
| CpG_4 | −0.13 (−0.23 to −0.021) | 0.019 | −0.11 (−0.21 to −0.0058) | 0.039 | −0.089 (−0.19 to 0.016) | 0.096 |
| CpG_5 | −0.049 (−0.14 to 0.037) | 0.26 | −0.047 (−0.13 to 0.041) | 0.29 | −0.033 (−0.12 to 0.055) | 0.46 |
The β values are derived from linear regression models.
*Unadjusted analyses: F2RL3 methylation=intercept + β (respirable dust/calculated respirable dust/years working as welder).
†Full model: intercept + β (respirable dust) + β1 (age) + β2 (previous smoking) + β3 (passive smoking) + β4 (education) + β5 (current residence) + β6 (respirator use).
‡Full model: intercept + β (calculated respirable dust) + β1 (age) + β2 (previous smoking) + β3 (passive smoking) + β4 (education) + β5 (current residence). For the calculated respirable dust, use of a respirator was included in the calculation as described in the Materials and methods section.
§Adjusted analysis: intercept + β (years working as welder) + β1 (previous smoking).
¶Full model: intercept + β (years working as welder) + β1 (previous smoking) + β2 (passive smoking) + β3 (education) + β4 (current residence) + β5 (respirator use).