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. 2015 Dec 16;6:1119. doi: 10.3389/fpls.2015.01119

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Copyright © 2015 Lin, Lin, Lai, Liu, Chen, Zhang, XuHan and Lai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Longan TAS3 transcript. (A) Sequence alignment of DlTAS3 in D. longan and AT3G17185.1 (TAS3) in A. thaliana. Asterisk represents the consensus nucleotide; TAS3- tasiRNAs named as 5’D1+ (reads), 5’D2+ (reads), and so on, are highlighted in different colors. The miR390 cleavage site in TAS3 transcript is showed by red arrow. (B) Phylogenetic relationships among plant TAS3 sequences using the neighbor-joining method. Bootstrap values are in percentages. GenBank accession numbers for each sequence represented in the tree are as follows: A. thaliana (AtTAS3a, At3g17185; AtTAS3b, At5g49615; AtTAS3c, At5g57735), V. vinifera (FQ386573); Lotus japonicus (AK338955); Malus domestica (XR_524192); C. sinensis (XR_371831); Solanum lycopersicum (JX047545); O. barthii (GQ420228); Nicotiana tabacum (FJ804751); Prunus mume (XR_513520); P. trichocarpa (XM_006378492). (C) Sequence alignment between 5’D5+ (284) and 5’D6+ (478).