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Validation of tasiRNA guided cleavage of target genes DlARF3, -4 and mapping of the cleavage sites in target gene mRNAs. Numbers indicate the fraction of cloned PCR products. The B3 DNA binding domain (B3), Auxin_resp, and AUX_IAA, are highlighted in green, red, and blue in the ARF3 and -4 proteins, respectively. The tasiRNA complementary sequence in the DlARF3 and -4 mRNA and the corresponding amino acid sequences are shown.
