Table 1.
Primers used in pri-miR390 and TAS3 cloning.
Primer name | Primer sequence(5′–3′) | Tm/°C | Description |
---|---|---|---|
390-F | CTGTGTATAGAGACATACATGATGAG | 55 | RT-PCR |
390-R | ACCCATCAAAGATATATGATCTAGTAG | ||
390-5RACE1 | CATGAAACTCAGGATAGATAGCGCC | 55.6 | 5′-RACE |
390-5RACE2 | GTGGCGCTATCCCTGCTGAG | 56.6 | |
390-SP1 | CATGAAACTCAGGATAGATAGCGCC | 55.6 | Tail-PCR |
390-SP2 | CAACAGCTCATCATCATCATCATC | 52 | |
390-SP3 | GTGGCGCTATCCCTGCTGAG | 56.6 | |
390-SP4 | GAGCTCTCATGGTGAAGGTGG | 54.8 | |
390-SP5 | GTGGTGGAGACGGTCTTGTTG | 54.8 | |
TAS3-F | TTCTTGACCTTGTAAGGCCTT | 55 | RT-PCR |
TAS3-R | AGCTCAGGAGGGATAGAAG | ||
TAS3-3P1 | TTCTTGACCTTGTAAGGCCTT | 53 | 3′-RACE |
TAS3-3P2 | TTCCGTCCAACTCATCTTCTC | 56 | |
TAS3-5P1 | AAGATGAGTTGGACGGAAAC | 54 | 5′-RACE |
TAS3-5P2 | TTCTTAACGCGGGATCTTAC | 56 |