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. 2015 Oct 1;8(10):12400–12409.

Figure 3.

Figure 3

C-Myc regulates H19 promoter activity, depending on E-box element. A: Schematic of the H19-promoter-luciferase construct is depicted with locations of the E-box element and sequences of point mutation; B: Luciferase reporter assay on A549 cells cotransfected with luciferase constructs (mutant at E-box element) and pcDNA3.1-c-Myc or c-Myc-siRNA; **P < 0.01, compared with pcDNA3.1 group, relative luciferase activity in pcDNA3.1-c-Myc group had statistical difference, ##P < 0.01, compared with si-NC group, relative luciferase activity in c-Myc-siRNA group had statistical difference; C: ChIP-derived DNA was amplified by qRT-PCR using specific primers. The levels of qPCR products are expressed as a percentage of input DNA; **P < 0.01, compared with the lgG value, the percentage of c-Myc input had statistical difference in Non-transfected, pcDNA3.1-c-Myc and c-Myc-siRNA group, respectively; ##P < 0.01, compared with Non-transfected group, percentage of c-Myc input in pcDNA3.1-c-Myc had statistical difference; ▲▲P < 0.01, compared with pcDNA3.1-c-Myc group, percentage of c-Myc input in c-Myc-siRNA group had statistical difference.