Skip to main content
. 2015 Oct 1;8(10):12446–12454.

Figure 4.

Figure 4

miR-194 directly targeted MAP4K4 by binding toits 3’UTR. A. Immunohistochemistry showed an inverse relationship between the expression of miR-194 and MAP4K4 in HCC specimens (scale bar = 50 μm). B. Western blot analysis of the expression of MAP4K4 in indicated cells. C. Predicted miR-194 target sequences in 3’UTR of MAP4K4 is shown (solid lines indicate matching base pairs and crosses represent non-matching base pairs). D. miR-194-overexpressing and control cells were transfected with the luciferase reporter plasmid carrying the wild type/mutant 3’UTR sequences of MAP4K4. A dual-luciferase reporter system analysis was performed. Data are expressed as the mean ± SD. *, P<0.05 vs. control group, n = 5.