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Re-expression of MAP4K4 abrogated the effect of miR-194. HepG2 and Hep3B cells were infected with MAP4K4 and control adenovirus for 48 h. A. Expression of MAP4K4 protein was analysed by Western blot. B. Cell viability was measured by the MTT assay. C. Proliferation capacity was examined by 3H thymidine incorporation assay. D, E. The cell apoptosis and cycle progression was analysed by flow cytometry. Data are expressed as the mean ± SD. *, P<0.05 vs. control group, n = 5.
