Expression of HH signaling pathway-related proteins. A. mRNA expressions of SHH, Patched-1, SMO, Gli-1 and Gli-2 in each group; B. Relative density of protein level of SHH, Patched-1, SMO, Gli-1 and Gli-2 in each group; C. Western blotting analysis of signaling pathway-related proteins level. *: P<0.05, **: P<0.01, and ***: P<0.001 compared to the control group.