Figure 1.

MBMECs were incubated with oxLDL for 24 h, and the cell viability was examined by CCK8 assay (A). Cellular cytotoxicity was assessed by LDH production when the MBMECs were exposed to oxLDL for 24 h (B). MDA (C) and NO (D) concentrations were measured with ELISA assay. ROS concentration was analyzed by fluorescence intensity values (E). eNOS mRNA expression was determined by real-time PCR (F). Values were expressed as mean ± SD, n = 3 in each group. *P < 0.05, **P < 0.01, ***P < 0.001 versus control group. LDH lactate dehydrogenase, MDA malondialdehyde, NO nitric oxide, ROS reactive oxygen species, eNOS endothelial nitric oxide synthase.