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MBMECs were incubated with oxLDL (100 mg/L) for 24 h, SHH, Ptch1, Smo and Gli1 mRNA expression were determined by real-time PCR (A). SHH, Ptch1, Smo and Gli1 protein expression were determined by Western blotting (B). Values were expressed as mean ± SD, n = 3 in each group. *P < 0.05, **P < 0.01, ***P < 0.001 versus control group. SHH, sonic hedgehog; Smo, smoothened protein; Ptch1, patched 1; Gli1, glioma-associated oncogene homolog 1.
