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. 2015 Oct 1;8(10):12853–12860.

Figure 3.

Figure 3

miR-503 plays its suppressive role in CRC by directly targeting E2F3. A. The sequences of miR-503 against the wild-type (WT) or mutant (Mut) 3’-UTR of E2F3 predicted by Targetscan. B. SW480 cells were co-transfected with miR-503 mimics (miR-503) or its negative control (miR-NC) and wild-type (WT) or mutated (Mut) 3’-UTR of E2F3. The luciferase activities were examined 48 h after transfection. C. The protein levels of E2F3 in tranfected SW480 cells examined by western blot. D. Relative expression levels of E2F3 in SW480 cells transfected with E2F3-specific siRNA (si-E2F3) or its negative control (si-NC). E. Cell proliferation of transfected SW480 cells determined by CCK-8 assays. F. Cell apoptosis rate of transfected SW480 cells measured by flow cytometry. G. Cell cycle distribution of transfected SW480 cells measured by flow cytometry. Data are mean ± SD of three independent experiments. *P < 0.05.