Table 1.
Primers used to amplify regions of CYP2C19
| Primer name | Primer Sequence (5’-3’) | PCR product size (bp) |
|---|---|---|
| Promoter_F | GCCTGTTTTATGAACAGGATGA | 918 |
| Promoter_R | TAAGACAACCGTGAGCTTGC | |
| Exon1_F | ACAGAGTGGGCACTGGGACGA | 844 |
| Exon1_R | GGTCCTAAACCCACAGCTGCTTCC | |
| Exon2_3_F | TTGTCTGACCATTGCCTTGA | 833 |
| Exon2_3_R | TCTCAGCTTCAAACCCTGCT | |
| Exon4_F | CCCCAACTATTCTCACCCTTT | 916 |
| Exon4_R | AAAGTGTGAATTGAAGGACAAGC | |
| Exon5_F | TCAGGTTGTGCAAACTCTTTT | 908 |
| Exon5_R | CCTTCACTCACTTTTTGATGGA | |
| Exon6_F | ATGTTGGTAAGTATACAATGTGAGT | 386 |
| Exon6_R | TCACACCATTAAATTGGGACAGA | |
| Exon7_F | TTTTGATTGGAAATTTTAGTCCATT | 921 |
| Exon7_R | TCAGTTCTTTCCAAACTGACCT | |
| Exon8_F | GTCACTGGCCTTAAGCTCATGCCT | 718 |
| Exon8_R | CCCAGCCTAGGGGGTGAGGG | |
| Exon9_F | TGAGAGTAGGGGAGGTGAAGA | 907 |
| Exon9_R | GATGACGGGTCAGAAGAAGC | |
| 3’-UTR_F | ACGGATTTGTGTGGGAGAGGGC | 674 |
| 3’-UTR_R | AATGCTCAGCCAAAATAGCTTCCCT |