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Overexpression of CISD2 mRNA and protein in HCC cell lines. Western blotting (A) and qPCR (B) examined the expression of CISD2 mRNA and protein in HCC cell lines (Huh7, SK-Hep1, Hep3B, HepG2, MHCC-97L and SNU423) and human hepatocyte cells (L02). Expression levels were normalized against α-Tubulin, respectively. Error bars represent the standard deviation of the mean (SD) calculated from three parallel experiments. *P<0.05.
