Figure 4.

Downregulation of CISD2 repressed proliferation and tumorigenicity in vivo. Effect of CISD2 on tumor formation in nude mouse xenograft model. A. Subcutaneous tumor growth curve of CISD2 shRNA#1 and #2-transduced SK-Hep1 and HepG2 cells in nude mice was compared with ShRNA-vector SK-Hep1 and HepG2 cells in nude mice (n=6). Tumor volumes were measured on the indicated days. B. A representative picture of tumor growth in nude mice subcutaneously inoculated with ShRNA-vector, CISD2 shRNA#1 and #2-transduced, SK-Hep1 and HepG2 cells. C. Mean tumor weights. Each bar represents the mean ± SD of three independent experiments. *P<0.05.