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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Structure. 2015 Nov 19;23(12):2256–2266. doi: 10.1016/j.str.2015.10.011

Table 2.

Dissociation rate constant(Kd) for WT RNase A and A109G variant determined for the binding of single nucleotide product analogs 3′-UMP and 5′-AMP at 25°C.

Kd (μM) Kd (μM)
3′-UMP 5′-AMP
WT 67 ± 6 105 ± 1
A109G 50 ± 8 440 ± 26