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. 2015 Dec 16;5:17887. doi: 10.1038/srep17887

Figure 1. TLR expression in NHA cells under basal conditions.

Figure 1

(a) mRNA expression of TLR1-10 at basal conditions of NHA (left) and positive controls provided with the kit (right). (b) Confocal microscopy for detection of TLR3, TLR4, and TLR5. For surface staining, cells were incubated on ice with TLR antibodies followed by cy5-conjugated F(ab)2 goat anti-rabbit IgG. For intracellular staining cells were fixed, permeabilized with saponin and then stained. FITC- phalloidin and DAPI were also used to label the culture, targeting filamentous actin and nuclear DNA, respectively. Bar scale: 10 μM.