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. 2015 Dec 16;5:17887. doi: 10.1038/srep17887

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IL-2-activated PBL were transferred to the upper chamber of the transwells and allowed to migrate in response to astrocytes under different conditions in the lower chamber for 4 h. Migrated cells were counted and stained with anti-CD3-PE, anti-CD19-ECD, anti-CD56-PC5, anti-CD4-PC7 and anti-CD8-FITC. The number of migrated CD4⁺T (a), NK cells (b), LB (c), and CD8⁺T (d) was counted and expressed as a percentage of the total migrated cells. The mean values (mean ± S.E.M.) of five independent experiments are shown.