Figure 2. Optimized xeno-free condition yields highly efficient production of neurons.

(a) Timeline of differentiation of hPSCs to neurons. (b) Upper panel: Pax6 + neuralepithial cells were observed from d10, with no significant differences in condition 1, 2, 3 and 4. Pax6 positive cells in condition 5 were significant decreased compared to other conditions. Lower panel: βIII-tublin + and FOXG1 + were expressed from d22, with similar levels in condition 1, 2, 3, and 4; while βIII-tublin + positive cells in condition 5 were significant decreased. Scale bar, 50 μm. (c) Quantifications of neuron numbers from each condition at d30 beginning with 1×10⁶ hPSCs. *p < 0.05. Data are presented as mean ± s.e.m. (d) Quantifications of βIII-tubulin + cells from different conditions. *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as mean ± s.e.m. (e) Quantifications of FOXG1 + cells from different conditions. *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as mean ± s.e.m.