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. 2015 Dec 16;5:18271. doi: 10.1038/srep18271

Figure 6. Treatment of PARylated PARP1 protein with SVP, EcRppH and HsNudT16 results in the identification of phosphoribosylation sites by mass spectrometry.

Figure 6

(a) E491 (red, identified as 212 Dalton mass shift) on PARP1 was identified as a PARP1 automodification site following SVP, EcRppH and HsNudT16 treatment. (b) E190 and K192 were identified as PARP1 automodification sites on PARP1 following EcRppH digestion. (c) Identification of R452 as a PARP1 automodification site following HsNudT16 treatment.